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Synthetic Biology

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Synthetic Biology

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Synthetic Biology: Should I use IRES or 2A elements to string CDSs together for polycistronic mammalian expression?

Synthetic Biology: What are the advantages and disadvantages of using Gibson Assembly vs. Golden Gate Assembly?

Synthetic Biology: Does CRISPRi or siRNA work better in mammalian cells for gene knockdown?

Synthetic Biology: Is there a way to reliably achieve single-copy genomic integration of genetic constructs in mammalian cells for mutant library screening?

Synthetic Biology: Is there a good way to express transgenes in bacteria without overwhelming them metabolically?

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